Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions
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Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions
Pyranose dehydrogenase (PDH) is a flavin-dependent sugar oxidoreductase that is limited to a rather small group of litter-degrading basidiomycetes. The enzyme is unable to utilize oxygen as an electron acceptor, using substituted benzoquinones and (organo) metal ions instead. PDH displays a broad substrate specificity and intriguing variations in regioselectivity, depending on substrate, enzyme...
متن کاملMannitol Dehydrogenase from Agaricus Campestris.
Enzymes capable of catalyzing n-mannitol formation from n-fructose or n-fructose 6-phosphate have been identified in various microorganisms. Substrate and coenzyme specificity have been established in only a few instances, but there appear to be enzymes, dependent upon nicotinamide adenine dinucleotide and upon nicotinamide adenine dinucleotide phosphate, which reduce fructose directly to manni...
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Monomeric Agaricus meleagris pyranose dehydrogenase (AmPDH) belongs to the glucose-methanol-choline family of oxidoreductases. An FAD cofactor is covalently tethered to His103 of the enzyme. AmPDH can double oxidize various mono- and oligosaccharides at different positions (C1 to C4). To study the structure/function relationship of selected active-site residues of AmPDH pertaining to substrate ...
متن کاملStudies on a hemagglutinin from the mushroom Agaricus campestris.
A phytohemagglutinin has been isolated in 80 to 90% purity from the mushroom Agaricus campestris. This protein had a sedimentation constant of 4.2 S and an electrophoretic mobility, at pH 8.6, remindful of the properties of a fast y-globulin. It was a nonspecific agglutinin reacting with erythrocytes and leukocytes from a number of species. The hemagglutination reaction was independent of tempe...
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Pyranose dehydrogenases (PDHs) are extracellular flavin-dependent oxidoreductases secreted by litter-decomposing fungi with a role in natural recycling of plant matter. All major monosaccharides in lignocellulose are oxidized by PDH at comparable yields and efficiencies. Oxidation takes place as single-oxidation or sequential double-oxidation reactions of the carbohydrates, resulting in sugar d...
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ژورنال
عنوان ژورنال: Biomolecules
سال: 2013
ISSN: 2218-273X
DOI: 10.3390/biom3030535